Histomorphological effects of valproic acid on the development of pancreatic islets in chick
Abstract
Objective: To determine the developmental histo-morphological effects of valproic acid on pancreatic islets by comparing the pancreatic islets of freshly hatched chicks with the pancreatic islets of chicks not receiving this medication intervention.
Study design: Experimental study.
Place and Duration of Study: At department of anatomy CPSP, Regional Centre, Islamabad, extending from February till November 2010.
Methodology: Two groups were made with thirty fertilized chicken eggs each, Group-A (experimental) and Group-B (control). Prior to incubation, Group-A's eggs were injected with valproic acid. Group-B eggs received a sham treatment using regular saline in same volume. On hatching, pancreata were removed and stained with Eosin and hematoxylin, orange G-light green and aldehyde fuchsin stain. The number of islets and the cells that make them, as well as the area of islets lobe-wise in the pancreatic splenic and third lobes were measured in each section. After statistical analysis, the collected data was contrasted with the control groups.
Results: The histo-morphological findings demonstrated that valproic acid inhibited the Pancreatic islets development in completely hatched chicks, resulting in a reduction in the quantity and area of the islets as well as in the number of component cells.
Conclusion: The formation of pancreatic islets and the cells that make them up is intensely suppressed by VPA. It causes hypotrophy and islet hypoplasia in newborns.
Key words: Pancreatic, valproic acid, chick embryo.
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Copyright (c) 2025 Lubna Akhtar, Nadia Rashid, Syed Muhammad Aleem Haider , Syed Zulifqar Haider , Nausheen Jamshed , Ruqqia Shafi Minhas
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