Histomorphological effects of valproic acid on the development of pancreatic islets in chick

Authors

  • Lubna Akhtar Foundation university school of health sciences Islamabad
  • Nadia Rashid Anatomy
  • Syed Muhammad Aleem Haider
  • Syed Zulifqar Haider
  • Nausheen Jamshed
  • Ruqqia Shafi Minhas

Abstract

Objective: To determine the developmental histo-morphological effects of valproic acid on pancreatic islets by comparing the pancreatic islets of freshly hatched chicks with the pancreatic islets of chicks not receiving this medication intervention.

Study design: Experimental study.

Place and Duration of Study: At department of anatomy CPSP, Regional Centre, Islamabad, extending from February till November 2010.

Methodology: Two groups were made with thirty fertilized chicken eggs each, Group-A (experimental) and Group-B (control). Prior to incubation, Group-A's eggs were injected with valproic acid. Group-B eggs received a sham treatment using regular saline in same volume. On hatching, pancreata were removed and stained with Eosin and hematoxylin, orange G-light green and aldehyde fuchsin stain. The number of islets and the cells that make them, as well as the area of islets lobe-wise in the pancreatic splenic and third lobes were measured in each section. After statistical analysis, the collected data was contrasted with the control groups.

Results: The histo-morphological findings demonstrated that valproic acid inhibited the Pancreatic islets development in completely hatched chicks, resulting in a reduction in the quantity and area of the islets as well as in the number of component cells.

Conclusion: The formation of pancreatic islets and the cells that make them up is intensely suppressed by VPA. It causes hypotrophy and islet hypoplasia in newborns.

Key words: Pancreatic, valproic acid, chick embryo.

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Published

2026-01-01

How to Cite

1.
Akhtar L, Rashid N, Haider SMA, Haider SZ, Jamshed N, Shafi Minhas R. Histomorphological effects of valproic acid on the development of pancreatic islets in chick. Adv Basic Med Sci [Internet]. 2026Jan.1 [cited 2026Jan.5];9(2):82-7. Available from: https://abms.kmu.edu.pk/index.php/abms/article/view/437

Issue

Vol. 9 No. 2 (2025): (July-Dec) Advances in Basic Medical Sciences

Section

Original Articles

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Copyright (c) 2025 Lubna Akhtar, Nadia Rashid, Syed Muhammad Aleem Haider , Syed Zulifqar Haider , Nausheen Jamshed , Ruqqia Shafi Minhas

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