How to Cite

Tariq, S. A. (2017). ELEMENTAL ANALYSIS OF INDIGOFERA GERARDIANA WALL BY ATOMIC ABSORPTION SPECTROPHOTOMETER (AAS). Advances in Basic Medical Sciences, 1(1). Retrieved from https://abms.kmu.edu.pk/index.php/abms/article/view/32


Background: Increase in frequency of environmental pollution particularly soil con-

taminated with heavy metals direct their uptake in human food through plant parts.

Tissue precipitation of macro-nutrients and micro-nutrients during utilization of

produces adversely affects one’s health. The current study is directed to assess

and quantify the presence of metallic elements in whole plant of Indigofera ge-


Methodology: The fresh specimen of Indigofera gerardiana Wall was collected from upper Dir. The sample of the plant was dried under shadow, cut into small pieces and grinded to coarse powder. Powder was then used to analyse for mac-ro nutrients by flame photometry and for micronutrients by atomic absorption spectrophotometer.

Results: The window of acceptability for metallic ion is being exceeded by Fe, Ni, Cr & Co in certain parts of plants but normal in others. Mostly, the micro-nutrients detected in leaves were beyond normal limits.

Conclusions: Our study showed the presence of substantial concentrations of micro- and macro-nutrients in samples of root, leaves and stem.



Hussain J, Khan FU, Ullah R, Muhammad Z, Rehman N, Shinwari ZK, et al. Nutrient evaluation and elemental analysis of four selected medicinal plants of Khyber Pa-khtoonkhwa, Pakistan. Pak J Bot. 2011;43(1):427-34.

Saeed M, Muhammad N, Khan H. Assessment of Heavy Metal Content of Branded Pakistani Herbal Prod-ucts. Tropical Journal of Pharmaceutical Research. 2011;10(4):499-506.

Shinwari ZK, M. Rehman, T. Watanabe and Y. Yoshikawa. Medicinal and Aromatic Plants of Pakistan (A Pictorial Guide). Kohat, Pakistan: Kohat University of Science and Technology; 2006.

Hamayun M, Khan A, Khan MA. Common medicinal folk recipes of District Buner, NWFP, Pakistan. Ethnobotani-cal Leaflets. 2003;2003(1):14.

Sittie AA, Nyarko AK. Indigofera arrecta: safety evalua-tion of an antidiabetic plant extract in non‐diabetic hu-man volunteers. Phytotherapy Research. 1998;12(1):52-4.

Nisar M, Tariq SA, Marwat IK, Shah MR, Khan IA. Anti-bacterial, antifungal, insecticidal, cytotoxicity and phytotoxicity studies on Indigofera gerardiana. Jour-nal of enzyme inhibition and medicinal chemistry. 2009;24(1):224-9.

Amala BE, Ganga N, Arivudainambi R, Santhanam

G. Anti-inflammatory activity of Indigofera aspala-thoides Vahl. The Indian journal of medical research. 1982;76:115

Esimone C, Adikwu M, Muko K. Antimicrobial properties of< i> Indigofera dendroides leaves. Fitoterapia. 1999;70(5):517-20.

Dahot MU. Antibacterial and antifungal activity of small protein of< i> Indigofera oblongifolia leaves. Jour-nal of ethnopharmacology. 1999;64(3):277-82.

Khan MA, Ahmad I, Rahman IU. Effect of environmen-tal pollution on heavy metals content of Withania somnifera. Journal of the Chinese Chemical Society. 2007;54(2):339-43.

Ajasa AMO, Bello MO, Ibrahim AO, Ogunwande IA, Olawore NO. Heavy trace metals and macronutrients status in herbal plants of Nigeria. Food Chemistry. 2004;85(1):67-71.

Srivastava SK, Rai V, Srivastava M, Rawat A, Mehrotra S. Estimation of heavy metals in different Berberis species and its market samples. Environmental monitoring and assessment. 2006;116(1-3):315-20.

Saeed M, Khan H, Khan MA, Khan F, Khan SA, Muhammad

N. Quantification of various metals and cytotoxic profile of aerial parts of Polygonatum verticillatum. Pak J Bot.


Benoist Bd, McLean E, Egll I, Cogswell M. Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia: World Health Organization; 2008.

Beard JL. Iron biology in immune function, muscle me-tabolism and neuronal functioning. The Journal of nutri-tion. 2001;131(2):568S-80S.

Cook JD. Diagnosis and management of iron-deficiency anaemia. Best Practice & Research Clinical Haematolo-gy. 2005;18(2):319-32.

Lukaski HC, Siders WA, Penland JG. Chromium picolinate supplementation in women: effects on body weight, composition, and iron status. Nutrition. 2007;23(3):187-95.

Tepper LB, Levin LS. A survey of air and population lead levels in selected American communities. Environmen-tal quality and safety Supplement. 1974;2:152-96.

Golub MS. Metals, fertility, and reproductive toxicity: CRC Press; 2005.

Schoeters G, Den Hond E, Dhooge W, Van Larebeke N, Leijs M. Endocrine disruptors and abnormalities of pu-bertal development. Basic & clinical pharmacology & toxicology. 2008;102(2):168-75.

Wang D, Du X, Zheng W. Alteration of saliva and serum concentrations of manganese, copper, zinc, cadmium

and lead among career welders. Toxicology letters. 2008;176(1):40-7.

Schwarz F, Kirchgessner M, Stangl G. Cobalt requirement of beef cattle—feed intake and growth at different lev-els of cobalt supply. Journal of Animal Physiology and Animal Nutrition. 2000;83(3):121-31.

Underwood E. Trace metals in human and animal health. International Journal of Food Sciences and Nutrition. 1981;35(1):37-48.

Morin Y, Tětu A, Mercier G. QUÉBEC BEER‐DRINK-

ERS’CARDIOMYOPATHY: CLINICAL AND HEMODYNAMIC ASPECTS*. Annals of the New York Academy of Sciences. 1969;156(1):566-76.

Basketter DA, Angelini G, Ingber A, Kern PS, Menné T. Nickel, chromium and cobalt in consumer products: re-visiting safe levels in the new millennium. Contact Der-matitis. 2003;49(1):1-7.

Shapiro R, Heaney R. Co-dependence of calcium and phosphorus for growth and bone development under conditions of varying deficiency. Bone. 2003;32(5):532-40.

Trumbo P, Yates AA, Schlicker S, Poos M. Dietary refer-ence intakes: vitamin A, vitamin K, arsenic, boron, chro-mium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. Journal of the Amer-ican Dietetic Association. 2001;101(3):294-301.