The Effect of Vitamin E on Glucose Levels in Diabetic Mice

Authors

  • Saadia Rashid Foundation University Medical College Islamabad.
  • Nadia Rashid Foundation Medical College, Foundation University Islamabad.
  • Sobia Islam Foundation Medical College, Foundation University Islamabad.
  • Lubna Akhtar Foundation Medical College, Foundation University Islamabad.
  • Nausheen Jamshed Karachi Institute of Medical Sciences, Karachi, Pakistan

DOI:

https://doi.org/10.35845/abms.2024.1.310

Abstract

Objective

To evaluate the blood glucose levels in diabetic mice treated with vitamin and those who were untreated.

Methods

It was an Experimental study. This study was conducted in the Anatomy Department of the Army Medical College, from November 2009 to November 2010 in cooperation with (NIH) National Institute of Health, Islamabad. Three groups of thirty mature female BALB/C mice were randomly selected. Group A acted as the study control. Streptozotocin (STZ) was administered intraperitoneally to Group B to cause diabetes. STZ was administered intravenously to Group C, and they had a diet high in Vitamin E (alphatocopherol). All Group’s Serum glucose levels were monitored using a glucometer at the beginning of the trial, after 72 hours following STZ injection, and then after 12 weeks.

Results                                                  

 There is a considerable change in blood glucose levels between the diabetic group (Group B) and a diabetic group treated with Vitamin E (Group C) respectively. When compared to experimental Group B, Group C’s blood glucose level dropped significantly. 

Conclusion

Vitamin E treatment for diabetic mice may have a significant impact on lowering blood glucose levels through the use of antioxidant therapy.

References

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes care. 2013 Jan 1;36(Supplement_1):S67-74.

Moreno JA, Gomez-Guerrero C, Mas S, Sanz AB, Lorenzo O, Ruiz-Ortega M, Opazo L, Mezzano S, Egido J. Targeting inflammation in diabetic nephropathy: a tale of hope. Expert opinion on investigational drugs. 2018 Nov 2;27(11):917-30.

Azeem S, Khan U, Liaquat A. The increasing rate of diabetes in Pakistan: A silent killer. Annals of medicine and surgery. 2022 Jul 1;79.

Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes research and clinical practice. 2010 Jan 1;87(1):4-14.

Oguntibeju OO. Type 2 diabetes mellitus, oxidative stress, and inflammation: examining the links. International journal of physiology, pathophysiology, and pharmacology. 2019;11(3):45.

Pourghasem M, Shafi H, Babazadeh Z. Histological changes of kidney in diabetic nephropathy. Caspian journal of internal medicine. 2015;6(3):120.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews. 2010 Jul;4(8):118.

Okoduwa SI, Umar IA, James DB, Inuwa HM. Appropriate insulin level in selecting fortified diet-fed, streptozotocin-treated rat model of type 2 diabetes for anti-diabetic studies. PLoS One. 2017 Jan 27;12(1):e0170971.

Wong DW, Oudit GY, Reich H, Kassiri Z, Zhou J, Liu QC, Backx PH, Penninger JM, Herzenberg AM, Scholey JW. Loss of angiotensin-converting enzyme-2 (Ace2) accelerates diabetic kidney injury. The American journal of pathology. 2007 Aug 1;171(2):438-51.

Sun C, Li X, Liu L, Canet MJ, Guan Y, Fan Y, Zhou Y. Effect of fasting time on measuring mouse blood glucose level. Int J Clin Exp Med. 2016 Jan 1;9(2):4186-9.

Tan GC, Tan SM, Phang SC, Ng YT, Ng EY, Ahmad B, Palamisamy UD, Kadir KA. Tocotrienol-rich vitamin E improves diabetic nephropathy and persists 6–9 months after washout: a phase IIa randomized controlled trial. Therapeutic advances in endocrinology and metabolism. 2019 Dec;10:2042018819895462.

Omar GM, Al-arabi FY, Mehdi MA, Fawade MM. Antioxidant effect of vitamins combined a, c, and e dose on type 2 diabetic patients. Asian Journal of Pharmaceutical and Clinical Research. 2019;12(4):51-4.

Rizzo MR, Abbatecola AM, Barbieri M, Vietri MT, Cioffi M, Grella R, Molinari A, Forsey R, Powell J, Paolisso G. Evidence for anti-inflammatory effects of combined administration of vitamin E and C in older persons with impaired fasting glucose: impact on insulin action. Journal of the American College of Nutrition. 2008 Aug 1;27(4):505-11.

Rizvi S, Raza ST, Ahmed F, Ahmad A, Abbas S, Mahdi F. The role of vitamin E in human health and some diseases. Sultan Qaboos University Medical Journal. 2014 May;14(2):e157.

Sharma M, Katyal T, Grewal G, Behera D, Budhiraja R. Effect of antioxidants such as β-carotene, vitamin C and vitamin E on oxidative stress, thermal hyperalgesia and cold allodynia in streptozotocin-induced diabetic rats. The Internet Journal of Pharmacology. 2008;6.

Rashid S. Effect of alphatocopherol on the diameter of proximal convoluted tubules of kidney in diabetic mice. JPMA. The Journal of the Pakistan Medical Association. 2014 Jan 1;64(1):46-9.

Wong SK, Chin KY, Suhaimi FH, Ahmad F, Ima-Nirwana S. Vitamin E as a potential interventional treatment for metabolic syndrome: evidence from animal and human studies. Frontiers in pharmacology. 2017 Jul 5;8:444.

Rashid S, Butt SA, Anjum S. Effects of Alpha-Tocopherol on Renal Glomeruli in Diabetic Mice. Journal of Rawalpindi Medical College. 2011 Jun 30;15(1).

Balbi ME, Tonin FS, Mendes AM, Borba HH, Wiens A, Fernandez-Llimos F, Pontarolo R. Antioxidant effects of vitamins in type 2 diabetes: a meta-analysis of randomized controlled trials. Diabetology & metabolic syndrome. 2018 Dec;10:1-2.

Khodaeian M, Tabatabaei‐Malazy O, Qorbani M, Farzadfar F, Amini P, Larijani B. Effect of vitamins C and E on insulin resistance in diabetes: a meta‐analysis study. European journal of clinical investigation. 2015 Nov;45(11):1161-74.

Rafighi Z, Shiva A, Arab S, Yusuf RM. Association of dietary vitamin C and E intake and antioxidant enzymes in type 2 diabetes mellitus patients. Global journal of health science. 2013 May;5(3):183.

Published

2024-06-21

How to Cite

Saadia Rashid, Rashid, N., Sobia Islam, Lubna Akhtar, & Nausheen Jamshed. (2024). The Effect of Vitamin E on Glucose Levels in Diabetic Mice. ADVANCES IN BASIC MEDICAL SCIENCES, 8(1), 23–27. https://doi.org/10.35845/abms.2024.1.310