Lower Calciferol Level In Diabetic People And Their Association With Glycated Haemoglobin


  • Rizwana Kalsoom Paharpur hospital Dera Ismail Khan
  • Muhammad Rehan Khan Cardiology Unit, DHQ Hospital Dera Ismail Khan
  • Saima Umer Mufti Memorial hospital DIKhan
  • Shahjahan Ustrana Department of Biochemistry, Gomal Medical College, Dera Ismail Khan
  • Irshad Ahmed Gomal Medical College D.I. Khan
  • Farhana Kalsoom Wensam College, Dera Ismail Khan




Calciferol, Adult Onset Diabetes Mellitus, Glycated haemoglobin, diabetes, vitamin D



To investigate the relationship between low calciferol levels and glycated hemoglobin in diabetics with normal blood sugar control.


Vitamin D (calciferol) levels and HbA1c levels were examined in 110 individuals with type 2 diabetes and a control group, ensuring both groups were similar in terms of gender and age. Calciferol (25(OH)D3) level was determined using the method of radioimmunoassay (RIA) and the High-performance liquid chromatography technique (HPLC) was used to determine the glycosylated hemoglobin (HbA1c) level.


Calciferol levels were found low in the test group (type 2 diabetic patients) 18.16 ± 0.85 ng/ml as compared to the control group 24.38 ±2.01ng/ml with (p≤0.000). The calciferol levels were assessed and it was found that they were inversely related to glycated hemoglobin levels in the test group (r (2) = 0.047, linear regression, p ≤ 0.009,). Calciferol levels were also inversely associated with glycated hemoglobin levels in combined analyzed data of test and control groups (p ≤ 0.001, r (2) = 0.079).


The study determined that the calciferol levels were found with decreased frequency in the diabetic group as compared to the control group and it showed that vitamin D levels have a key role in glycemic control in type 2 diabetic patients. These findings support the idea that type 2 diabetic patients may improve their glycemic control by using vitamin D supplementation.


Povaliaeva A, Pigarova E, Zhukov A, Bogdanov V, Dzeranova L, Mel’nikova O, Pekareva E, Malysheva N, Ioutsi V, Nikankina L, Rozhinskaya L. Evaluation of vitamin D metabolism in patients with type 1 diabetes mellitus in the setting of cholecalciferol treatment. Nutrients. 2020 Dec 18;12(12):3873.

El Lithy A, Abdella RM, El-Faissal YM, Sayed AM, Samie RM. The relationship between low maternal serum vitamin D levels and glycemic control in gestational diabetes assessed by HbA1c levels: an observational cross-sectional study. BMC pregnancy and childbirth. 2014 Dec;14:1-6.

George PS, Pearson ER, Witham MD. Effect of vitamin D supplementation on glycaemic control and insulin resistance: a systematic review and meta‐analysis. Diabetic medicine. 2012 Aug;29(8):e142-50.

Szymczak-Pajor I, Śliwińska A. Analysis of association between vitamin D deficiency and insulin resistance. Nutrients. 2019 Apr 6;11(4):794.

Holick MF. The vitamin D deficiency pandemic: Approaches for diagnosis, treatment and prevention. Reviews in Endocrine and Metabolic Disorders. 2017 Jun;18:153-65.

Siddiqee MH, Bhattacharjee B, Siddiqi UR, MeshbahurRahman M. High prevalence of vitamin D deficiency among the South Asian adults: a systematic review and meta-analysis. BMC Public Health. 2021 Dec;21:1-8.

Baxter JA, Wasan Y, Hussain A, Soofi SB, Ahmed I, Bhutta ZA. Characterizing Micronutrient status and risk factors among late adolescent and young women in rural Pakistan: A cross-sectional assessment of the Mapps trial. Nutrients. 2021 Apr 9;13(4):1237.

Ponmalar P. Effect of Serum 25 Hydroxy Cholecalciferol on Glycemic Status and Lipid Profile in Type 2 Diabetes Mellitus (Doctoral dissertation, Kilpauk Medical College, Chennai) 2019.

Palomer X, González‐Clemente JM, Blanco‐Vaca F, Mauricio D. Role of vitamin D in the pathogenesis of type 2 diabetes mellitus. Diabetes, Obesity, and Metabolism. 2008 Mar;10(3):185-97.

Lau SL, Gunton JE, Athayde NP, Byth K, Cheung NW. Serum 25‐hydroxyvitamin D and glycated hemoglobin levels in women with gestational diabetes mellitus. Medical journal of Australia. 2011 Apr;194(7):334-7.

Aghajafari F, Nagulesapillai T, Ronksley PE, Tough SC, O’Beirne M, Rabi DM. Association between maternal serum 25-hydroxyvitamin D level and pregnancy and neonatal outcomes: systematic review and meta-analysis of observational studies. Bmj. 2013 Mar 26;346.

Hoxha M, Zoto M, Deda L, Vyshka G. Vitamin D and its role as a protective factor in allergy. International scholarly research notices. 2014;2014(1):951946.

Cutolo M, Otsa K, Uprus M, Paolino S, Seriolo B. Vitamin D in rheumatoid arthritis. Autoimmunity reviews. 2007 Nov 1;7(1):59-64.

Tai K, Need AG, Horowitz M, Chapman IM. Glucose tolerance and vitamin D: effects of treating vitamin D deficiency. Nutrition. 2008 Oct 1;24(10):950-6.

Guasch A, Bulló M, Rabassa A, Bonada A, Del Castillo D, Sabench F, Salas-Salvadó J. Plasma vitamin D, and parathormone are associated with obesity and atherogenic dyslipidemia: a cross-sectional study. Cardiovascular diabetology. 2012 Dec;11:1-1.

Indhavivadhana S, Boonyachan W, Rattanachaiyanont M, Wongwananuruk T, Techatraisak K, Sa-Nga-Areekul N. Effectiveness of vitamin D2 supplementation on high-sensitivity C-reactive protein and other metabolic indices in menopausal Thai women: a randomized controlled trial. Gynecological Endocrinology. 2022 Jan 2;38(1):83-9.

Melguizo-Rodríguez L, Costela-Ruiz VJ, García-Recio E, De Luna-Bertos E, Ruiz C, Illescas-Montes R. Role of vitamin D in the metabolic syndrome. Nutrients. 2021 Mar 3;13(3):830.

Mozos I, Marginean O. Links between vitamin D deficiency and cardiovascular diseases. BioMed research international. 2015;2015(1):109275.

Brown AC. Kidney toxicity related to herbs and dietary supplements: Online table of case reports. Part 3 of 5 series. Food and Chemical Toxicology. 2017 Sep 1;107:502-19.

Pludowski P, Holick MF, Pilz S, Wagner CL, Hollis BW, Grant WB, Shoenfeld Y, Lerchbaum E, Llewellyn DJ, Kienreich K, Soni M. Vitamin D effects on musculoskeletal health, immunity, autoimmunity, cardiovascular disease, cancer, fertility, pregnancy, dementia and mortality—a review of recent evidence. Autoimmunity reviews. 2013 Aug 1;12(10):976-89.

Szymczak-Pajor I, Miazek K, Selmi A, Balcerczyk A, Śliwińska A. The action of vitamin D in adipose tissue: is there the link between vitamin D deficiency and adipose tissue-related metabolic disorders?. International Journal of Molecular Sciences. 2022 Jan 16;23(2):956.

Park CY, Han SN. The role of vitamin D in adipose tissue biology: adipocyte differentiation, energy metabolism, and inflammation. Journal of Lipid and Atherosclerosis. 2021 May;10(2):130.



How to Cite

Kalsoom, R., Khan, M. R. ., Umer, S. ., Ustrana, S. ., Ahmed, I. ., & Kalsoom, F. . (2024). Lower Calciferol Level In Diabetic People And Their Association With Glycated Haemoglobin. ADVANCES IN BASIC MEDICAL SCIENCES, 8(1), 12–16. https://doi.org/10.35845/abms.2024.1.298